|本期目录/Table of Contents|

大鼠呼吸系统表面活性蛋白SP-A和SP-D分泌细胞的分布研究(PDF)

《内蒙古大学学报(自然科学版)》[ISSN:1000-9035/CN:22-1262/O4]

期数:
2019年06期
页码:
62-67
栏目:
研究论文
出版日期:
2019-11-15

文章信息/Info

Title:
Distribution of Surfactant Proteins A and D Secreting Cells in Rat Respiratory System
作者:
齐旺梅1徐斯日古楞1蔡 硕2金 凤1曹贵方1
1.内蒙古农业大学兽医学院,呼和浩特 010018;
2.中国农业科学院上海兽医研究所 农业农村部兽用化学药物及制剂重点实验室,上海 200241
Author(s):
QI Wang-mei1XU Siriguleng1CAI Shuo2JIN Feng1CAO Gui-fang1
1.College of Veterinary Medicine,Inner Mongolia Agricultural University,Hohhot 010018,China;
2.Key Laboratory of Veterinary Chemical Drugs and Pharmaceutics,Ministry of Agriculture and Rural Affairs/Shanghai Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Shanghai 200241,China
关键词:
呼吸系统 表面活性蛋白 SP-A SP-D
Keywords:
respiratory system surfactant protein SP-A SP-D
分类号:
-
DOI:
-
文献标识码:
A
摘要:
为了揭示表面活性蛋白SP-A和SP-D分泌细胞在呼吸系统中的分布.使用大鼠鼻腔到肺的各部位组织冰冻切片,进行免疫组织化学染色.结果显示,从鼻腔到小支气管的小颗粒细胞和杯状细胞细呈SP-D阳性; 在喉腺和气管腺及其导管上皮呈SP-D强阳性; 在细支气管克拉拉细胞和肺Ⅱ型细胞均呈SP-A和SP-D阳性.揭示了在大鼠呼吸系统中SP-D分泌细胞比SP-A分泌细胞更广泛分布,SP-A与SP-D对于维持肺部无菌环境起到重要免疫防御作用.
Abstract:
To study the distribution of the surfactant proteins A and D secreting cells in rat respiratory system,tissues from nasal cavity to lungs were sampled and made into frozen sections for immunohistochemical (IHC) staining.The results demonstrated that,SP-D was positive stained in small granulosa cells and goblet cells from the nasal cavity to the small bronchus and positive stained in the larynx and trachea.The Laryngeal gland and tracheal gland and its ductal epithelium were strongly positive stained for SP-D.SP-A and SP-D were positive stained in Bronchial Clara cell and lung type II cells.In conclusion,SP-D secreting cells distributed more widely than SP-A secreting cells in rat respiratory system and they may played important roles in the immune and defense in the lung to keep an aseptic environment.

参考文献/References

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备注/Memo

备注/Memo:
收稿日期:2019-07-01; 修回日期:2019-08-27
基金项目:国家留学基金(西部地区人才培养特别项目)资助项目
作者简介:齐旺梅(1974-),女(蒙古族),内蒙古呼和浩特人,讲师,博士.主要从事动物组织与胚胎学,粘膜免疫学研究.E-mail: qiwangmei@imau.edu.cn
通信作者:曹贵方(1963-),男,内蒙古呼和浩特人,教授,博士.主要从事动物组织胚胎与发育生物学,分子发育生物学.E-mail:guifangcao@126.com
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