|本期目录/Table of Contents|

蒙古口蘑菌丝体凝集素性质初步研究(PDF)

《内蒙古大学学报(自然科学版)》[ISSN:1000-9035/CN:22-1262/O4]

期数:
2014年01期
页码:
38-42
栏目:
研究论文
出版日期:
2014-02-25

文章信息/Info

Title:
A Primary Study on Characteristics of Lectin from Mycelia of Tricholoma Mongolicum
作者:
孟建宇1 杨燕1 刘佳杰1 郝建华2
1. 内蒙古农业大学生命科学学院, 呼和浩特 010018;
2. 中国水产科学研究院黄海水产研究所, 山东青岛 266071
Author(s):
MENG Jian-yu1 YANG Yan1 LIU Jia-jie1 HAO Jian-hua2
1. College of Life Sciences, Inner Mongolia Agricultural University, Hohhot 010018, China;
2. Yellow Sea Fisheries Research Lnsititute, Chinese Academy of FisherySciences, Qingdao 266071, China
关键词:
蒙古口蘑菌丝体凝集素糖抑制稳定性
Keywords:
Tricholoma mongolicummycelialectinsaccharide inhibitionstability
分类号:
Q935
DOI:
-
文献标识码:
-
摘要:
通过液体摇瓶培养蒙古口蘑菌丝体,用磷酸盐缓冲液浸提,得到菌丝体的凝集素粗提物.分析表明:蒙古口蘑菌丝体凝集素对牛血和羊血都能凝集;D-果糖、β-葡萄糖、半乳糖和木糖对菌丝体凝集素均具有抑制作用;弱酸或弱碱性浸提液有利于凝集素的提取;菌丝体凝集素具有一定的热稳定性,直到60℃以后凝集红细胞的活力才丧失;其凝集活性对Ca2+、Mg2+、Mn2+和Fe3+这四种离子有不同程度的依赖.
Abstract:
The mycelia of Tricholoma mongolicum were cultured by the shake-flask cultivation.The lectin was extracted from mycelia of Tricholoma mongolicum by phosphate buffer and its properties were tested and analyzed.The results showed that the lectin was able to agglutinate cattle erythrocytes and sheep erythrocytes.The agglutination activity could be inhibited by D-fructose,β-glucose,galactose and xylose.It was beneficial to extract the lectin in the weak acidic or weak alkaline buffer.The lectin was stable in heat treatment,and it still has partial activity at 60℃.Metal ions were indispensable for the agglutination activity of lectin,the effects of Ca2+,Mg2+,Mn2+and Fe3+are varied.

参考文献/References

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备注/Memo

备注/Memo:
收稿日期:2013-4-27;改回日期:2013-10-8。
基金项目:内蒙古自治区自然科学基金资助项目(2010BS0506)
作者简介:孟建宇(1977-),男,内蒙古乌兰察布人,讲师,硕士.Email:meng_jianyu@imau.edu.cn
更新日期/Last Update: 1900-01-01